Figure 3.

Impact of the high density of dsDNA links or motor molecules on bead excursions. (a) A diagram illustrates the four examined groups of kinesin-1 beads, which had different coatings. The length and density of dsDNA links and the density of kinesin-1 were varied to study the impact of these factors on bead excursions measured with the TCM assay. (b and c) Run length (b) and velocity (c) were measured for kinesin-1 beads; see (a). Data show the mean ± SEM measured at 30 μM Mg-ATP; n = 29, 40, 10, and 18 beads for groups 1, 2, 3, and 4, respectively. (d) Experimentally measured SD of Y’-excursions are shown based on n = 29, 56, 20, and 28 beads for groups 1, 2, 3, and 4, respectively. Error bars (SD) were generated by bootstrap analysis. To see this figure in color, go online.