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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 May 18;142(3):984–987.e6. doi: 10.1016/j.jaci.2018.05.004

FIG 2.

FIG 2

Flow cytometry gating strategy used to quantify CD4+ IL-4 GFP+ T-cells (a). Percentage of IL-4-expressing CD4+ T-cells of mice immunized with rBet v 1 or BPE (b). Comparison of nBet v 1-depleted and rBet v 1-replaced extracts with untreated BPE (c); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. Schematic overview of nBet v 1-depletion of BPE using immunoprecipitation (d). mRNA expression of in vitro stimulated human naïve CD4+ T-cells with BPE (e).