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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Brain Behav Immun. 2018 Jul 3;73:584–595. doi: 10.1016/j.bbi.2018.07.002

Figure 3. KCa3.1 channels modulate microglia-motor neuron interaction in hSOD1G93A mice.

Figure 3

A: top, treatment scheme. Bottom, quantification of MNs (counted as SMI-32 stained cells in the ventral horns of the spinal cord) in non-tg wt, and hSOD1G93A mice treated with vehicle or TRAM-34. Data are shown as mean ± SEM ***p<0.001 vs wt; ###p<0.001 vs vehicle by One Way ANOVA, TRAM-34 n=6; Vehicle n=5; wt n=3). Representative immunofluorescence images are shown on the right (scale bar=20µm). B Non-tg wt MNs co-cultured with microglia isolated from the spinal cord of wt, symptomatic or end-stage hSOD1G93A mice treated with TRAM-34 (2.5 µM, black bars) or DMSO (white bars). After 72 h, MN death was quantified counting the activated caspase 3/SMI32 double positive cells. Data are mean ± SEM; n=3 in triplicate, °°°p<0.001 vs wt microglia, ***p<0.001 vs symptomatic microglia, ###p<0.001 vs end-stage microglia, @@@p<0.001 vs wt microglia+TRAM-34 by Two Way ANOVA (Holm-Sidak method): Representative immunofluorescence images are shown on the right (scale bar=20µm).