Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Brain Behav Immun. 2018 Jun 20;73:520–532. doi: 10.1016/j.bbi.2018.06.017

Figure 3. Sustained stimulation of β2- and β3ARs results in increased microglia and astrocyte activation on days 14 and 21.

Figure 3

Quantitative analysis of immunofluorescence intensity demonstrates that rats receiving sustained delivery of OR486 for 14 days, exhibit increased activity of (A) CD11b+ microglia and (B) GFAP+ astrocytes in the spinal dorsal horn on days 14 and 21. OR486-induced activation of microglia and astrocytes is blocked by co-administration of ICI-118,551 + SR59230A on day 0. Day 14 groups: Vehicle/Vehicle n=5 (3 males + 2 females); OR486/Vehicle n=6 (3 males + 3 females); OR486/ICI&SR n=6 (3 males + 3 females); Vehicle/ICI&SR n=5 (3 males + 2 females). Day 21 groups: Vehicle/Vehicle n=6 (3 males + 3 females); OR486/Vehicle n=7 (4 males + 3 females); OR486/ICI&SR n=7 (4 males + 3 females); Vehicle/ICI&SR n=6 (3 males + 3 females). Data are expressed as mean ± SEM. **P < 0.01 versus vehicle/vehicle.