Figure 4.

Intensity plots from stopped-flow experiments at three protein concentrations. (A) Initial signal intensities of tetramers in tetramer buffer are shown as a function of the protein concentration in arbitrary units (AU). Open squares: mean values of the tetramer intensity signals. The error bars designate the plus-minus SDs from the means. Filled squares: values obtained from the global fit to all data points for the given protein concentration. (B) Time-dependent changes of the scattering intensities are shown in arbitrary units (AU) after addition of assembly start buffer to the three protein concentrations depicted in (A). Mean values were calculated from the measurements at five successive time points and plotted as follows: lower curve: 0.05 g/L; middle curve: 0.10 g/L; upper curve: 0.20 g/L. The lines represent values obtained by a global fit to the whole set of data obtained for the three protein concentrations. (C) Residuals of the scattering curves shown in (B). The upper, middle, and lower panels relate to the upper, middle, and lower curves shown in (B). To see this figure in color, go online.