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. 2018 May 10;114(10):2408–2418. doi: 10.1016/j.bpj.2018.04.032

Table 1.

Determination of Rate Constants

c [g/L] ʎ [nm] k1a k2a k3a k4a χ2b
0.05/0.1/0.2 436 158 128 13 1.1 3/6/3
0.05/0.1/0.2c 436 179 118 15 0.5 4/6/6
0.4 365/436/594 253 109 16 0.5 5/8/13
0.4d 594 191 116 16 0.5 8/7/5
0.6e 594 214 97 13 0.4 5/5
Mean ± SD 199 ± 33 114 ± 10 14.6 ± 1.4 0.6 ± 0.3
a

Global fitting-derived rate constants in μM−1s−1.

b

χ2, chi-squared test.

c

Results do not change significantly if k4 is set to 0.5 μM−1s−1, as determined at the higher protein concentrations listed below.

d

3 × independent measurements.

e

2 × independent measurements.