Figure 2.

Biophysical analysis and comparison of the CTPR, CTPR-Y91D, and CTPR-Y91D-loop series of proteins. (a) Far-UV CD spectra, (b and c) averaged equilibrium denaturation curves monitored by (b) CD at 222 nm and converted to molar ellipticity and (c) fluorescence converted to fraction unfolded for ease of comparison, and (d) chevron plots are shown. The denaturation curves are fitted to a two-state model. The chevron plots are fitted to a two-state model in which folding and unfolding reaction proceed via a broad transition-state model. Measurements were performed at 25°C in 50 mM sodium phosphate buffer (pH 6.8), 150 mM NaCl. To see this figure in color, go online.