Table 1.
Parameters Obtained by Fitting the Equilibrium Denaturation Data to a Two-State Model for the CTPR, CTPR-YD, and the CTPR6-YD-Loop Proteins Series
| Experiment/Protein | D50% (M) | m-Value (kcal mol−1 M−1) | (kcal mol−1) |
|---|---|---|---|
| Equilibrium Denaturation Monitored by Fluorescence | |||
| CTPR2 | 3.53 ± 0.01 | 2.09 ± 0.04 | −7.4 ± 0.1 |
| CTPR3 | 4.30 ± 0.02 | 2.8 ± 0.2 | −12.0 ± 0.9 |
| CTPR4 | 4.80 ± 0.01 | 4.0 ± 0.3 | −19.2 ± 0.9 |
| CTPR6 | 5.30 ± 0.03 | 4.8 ± 0.2 | −25.5 ± 1.1 |
| CTPR2-YD | 2.28 ± 0.01 | 2.1 ± 0.04 | −4.8 ± 0.1 |
| CTPR3-YD | 3.93 ± 0.02 | 2.9 ± 0.2 | −11.4 ± 0.8 |
| CTPR6-YD-loop10 | 4.35 ± 0.01 | 3.4 ± 0.1 | −14.9 ± 0.6 |
| CTPR6-YD-loop15 | 4.32 ± 0.02 | 3.1 ± 0.3 | −13.4 ± 1.1 |
| CTPR6-YD-loop20 | 4.37 ± 0.02 | 3.4 ± 0.3 | −14.9 ± 1.3 |
| CTPR6-YD-loop25 | 4.38 ± 0.02 | 3.1 ± 0.2 | −13.6 ± 0.9 |
| CTPR6-YD | 4.99 ± 0.03 | 4.5 ± 0.5 | −22.5 ± 2.3 |
| Equilibrium Denaturation Monitored by CD | |||
| CTPR2 | 3.50 ± 0.01 | 2.2 ± 0.03 | −7.6 ± 0.1 |
| CTPR3 | 4.46 ± 0.01 | 3.3 ± 0.04 | −10.3 ± 0.1 |
| CTPR4 | 4.85 ± 0.01 | 4.8 ± 0.1 | −23.3 ± 0.5 |
| CTPR6 | 5.41 ± 0.01 | 4.9 ± 0.1 | −26.5 ± 0.5 |
| CTPR2-YD | 2.32 ± 0.02 | 1.8 ± 0.1 | −4.2 ± 0.1 |
| CTPR3-YD | 3.96 ± 0.01 | 2.31 ± 0.03 | −9.6 ± 0.1 |
| CTPR6-YD-loop10 | 4.19 ± 0.01 | 2.69 ± 0.06 | −11.3 ± 0.3 |
| CTPR6-YD-loop15 | 4.24 ± 0.01 | 2.64 ± 0.04 | −11.2 ± 0.2 |
| CTPR6-YD-loop20 | 4.21 ± 0.01 | 3.08 ± 0.04 | −12.9 ± 0.2 |
| CTPR6-YD-loop25 | 4.20 ± 0.01 | 2.88 ± 0.04 | −12.1 ± 0.2 |
| CTPR6-YD | 4.97 ± 0.01 | 4.3 ± 0.1 | −21.3 ± 0.4 |
All measurements were performed in triplicate, and the errors listed are the SE of the mean. The for the loop-extension proteins are apparent values only, as their low m-values indicate that the unfolding transitions are not fully cooperative.