Figure 3.

ONC201 decreased adhesion and invasion in USC cells. The ARK1 and SPEC-2 cell lines were cultured for 24 hours and then treated with ONC201 in a laminin-coated 96 well plate for 2 hours to assess adhesion (A) or a BME coated 96 transwell plate for 4 hours to assess invasion (B), respectively. The data represents relative inhibition in each cell line. Wound healing assay showed that ONC201 increased wound width in both cells in a dose-dependent manner (C). VEGF, Snail, Slug, E-Cadherin and N-cadherin were measured by Western blotting in cell lysates after 24 hours exposure to ONC201 (D). Each experiment was performed three times (*P<0.05, **P<0.01).