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. 2018 Aug 1;8(8):1414–1426.

Figure 1.

Figure 1

hZIP1 is a direct target of miR-223. A. The putative miR-223 binding site in the 3’-UTR region of hZIP1. B. 786-O cells were cotransfected with miR-223 (or mim-NC) and hZIP1-WT (or hZIP1-MUT) 3’-UTR luciferase reporter constructs, and the luciferase activity was tested. C. hZIP1 mRNA levels were examined by qRT-PCR. D. Protein levels of hZIP1 were detected by western blot in ccRCC cells transfected with the miR-223 mimic. mim-NC, mimic negative control; *P<0.05, **P<0.01 compared with the control group.