Figure 5.

IL8 attenuates the effects of miR-506 on migration, invasiveness, and EMT of HCC cells. HCCLM3 and MHCC97H cells transfected with miR-506 mimics or miR-ctrl were treated with 100 pg/mL recombinant IL8 protein. A. A wound-healing assay was performed to assess the cell migration ability. B. A Transwell invasion assay was conducted to evaluate cell invasion. C. The expression levels of E-cadherin and vimentin were assessed by western blotting. β-actin served as an internal control. *P < 0.05 as compared with the miR-ctrl group; &P < 0.05 as compared with the miR-506 group.