Figure 2.

KM8094 suppresses glutamine dependent cell growth in gastric cancer cells. (A) Seven lines of gastric cancer cells (HSC-39, MKN-1, SNU-16, HSC-40A, MKN28, 60As6, and HSC-60) were incubated in the medium containing of 0, 0.2, or 2 mM Gln for 96 h. Time course of growth for each cell line was assessed. Data are presented as means + SD of triplicate readings. (B-E) Effect of ASCT2 knockdown on SNU-16 and MKN28 cells. Cells were transfected with ASCT2 siRNA or nonspecific siRNA control. (B, C) Expression of ASCT2 mRNA (B) and protein (C) in SNU-16 and MKN28 cells after 48, 72, and 144 h of siRNA treatment. (D) After 72 and 144 h of siRNA treatment, the transfected cells were harvested and incubated with ¹⁴C-Glutamine for 15 min. The intracellular ¹⁴C-Glutamine level was assessed. Data are presented as means + SD of triplicate measurements. (E) After 48 h of siRNA treatment, the transfected cells were incubated in the medium containing 0.2 mM Gln for 72 h and the cell growth was assessed. Data are presented as means + SD of triplicate measurements. (F) Cells were incubated with 10 μg/mL KM8094 or KM8047 (control antibody) in the medium containing of 0.2 mM Gln for 72 h, after which the cell growth was assessed. Data are means + SD from three independent experiments.