Figure 4.

Effects of pharmacological modulation of apoptosis and necroptosis on NDV-induced CRT exposure. A549 (A) and H460 (B) lung cancer cells were pre-treated with either Z-VAD-FMK (100 μM) or Necrostain-1 (Nec-1, 20 μM), and subsequently infected or mock-infected with NDV/FMW (MOI = 1) for 24 h. Cells were stained for the detection of CRT in viable, PI-negative cells (Figure 2A). Representative dot plots (top panel) and quantification data (lower panel) are shown for three independent experimental replicates (***P<0.001, n.s = not significant).