Figure 4.

TOB1-AS1 sponged miR-27b with complementary binding at 3’-UTR. A. Schematic diagram showed the complementary bound within miR-27b and TOB1-AS1 3’-UTR with binding sites. B-D. The expression levels of miR-27b were tested after up- or downregulation of TOB1-AS1 in cervical cancer cells. E. The expression of TOB1-AS1 was evaluated. F. Luciferase reporter assay was performed in HeLa cells transfected with TOB1-AS1 wild (or mutant) type and miR-27b mimics (or mimic control). mim con, mimic control; inh con, inhibitor control. G. The co-localization between TOB1-AS1 and miR-27b in HeLa cells. *P<0.05; **P<0.01.