Figure 1.
Examples of typical auto- (green dashed/red dotted lines) and cross correlation (blue solid lines) curves. The lines are the averages, and the shadowed polygons show the SD of the correlation function calculated at each pixel. The upper panel shows the average cross correlation and SD of laminA-eGFP with H2A-mRFP together with their respective ACFs corresponding to one nonbinned pixel selected in a sample cell. The lower panel reports the cross correlation function and the SD of eGFP4x with H2A-mRFP next to the ACFs of each of them at a selected single pixel (a pixel) of a control cell. Both sample cells and controls are LMNA−/− MAF-H2A-mRFP transiently transfected with lamin A-eGFP and GFP-4x, respectively. The horizontal dashed line indicates the cross talk level between green and red channels. To see this figure in color, go online.