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Parameter images of SPIM-FCCS measurement of lamin A-eGFP and H2A-mRFP. The distribution of the lamin A (green) and the H2A (red) fluorescence signals can be seen in the left panel (the analog-to-digital converter unit (ADU) is the output unit of the EMCCD camera). The map of the relative CCF amplitude in the nucleus of LMNA−/− MAF-H2A-mRFP transiently transfected with lamin A-eGFP is shown in the right panel. Negative values of q are originated by the instrumental noise.
