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. 2018 Aug 15;10(8):2579–2589.

Figure 3.

Figure 3

miR-7702 directly targets TADA1 in human colorectal cancer cells. A. A schematic representation of miR-7702 target sites in the 3’ UTR of human TADA1. The artificial mutant 3’ UTR lacks these miR-7702 binding sites. B. Dual luciferase-reporter assays showed that miR-7702 significantly reduced the luciferase activity of reporter vectors containing WT predicted target sites (TADA1-wt-3’ UTR) but had little effect on the mutant group (TADA1-mut-3’ UTR). C. qRT-PCR assays showed that miR-7702 overexpression decreased endogenous TADA1 mRNA levels. GAPDH served as an internal reference. *P < 0.05. Data are representative of three independent experiments (means ± SD.). D. Western blot analyses showed that miR-7702 overexpression reduced endogenous TADA1 protein levels. GAPDH served as an internal control.