Figure 4.

miR-7702 inhibits the migration and invasion of human colorectal cancer cells. A. SW620 cells were co-transfected with miR-7702 mimics, pCMV6-control, or pCMV6-TATA1 for 48 h. Western blot analyses were performed to examine the protein expression levels of TADA1. B. miR-7702 overexpression inhibited the migration and invasion of SW620 cells. Migration and invasion was assayed in SW620 cells following co-transfection with miR-7702 minics, pCMV6-control, or pCMV6-TATA1 using the Transwell apparatus. Migrating or invading cells were stained with DAPI and analyzed under a microscope. C. Relative cell migration and invasion were determined based on the number of DAPI-stained SW620 cells. Cell migration and invasion are expressed as a percentage of that observed in control. D. Western blot analyses showed that miR-7702 inhibitor increased endogenous TADA1 protein levels. E. The miR-7702 inhibitor promoted the migration and invasion of SW480 cells. Migration and invasion were assayed in SW480 cells following transfection with the miR-7702 inhibitor. F. Relative cell migration are invasion were determined based on the number of DAPI-stained SW480 cells. G, H. Cell viability assay showed that miR-7702 had no effect on SW620 and SW480 cells proliferation. The line charts show relative CCK-8 absorbance, which indicates cellular viability. Data are the means ± SD. of three independent experiments. *P < 0.05 versus control.