Figure 1.

The cytotoxic effect of LiCl on MM cell survival. A, B. Indicated cells were seeded at 1 × 10⁴ cells per well in 96-well plates and cell proliferation detection of RPMI-8226 and U266 cells treated with LiCl in different concentrations (0, 5 mM, 10 mM, 20 mM, and 40 mM) for 48 h, with 40 mM NaCl used as a negative control. C, D. Indicated cells were seeded at 1 × 10⁴ cells per well in 96-well plates and CCK-8 analysis of RPMI-8226 and U266 cells exposed to 40 mM LiCl at different times (12, 24, 36, 48 and 72 h). E. BrdU incorporation assay, using RPMI-8226 cells that were treated with 40 mM LiCl for 48 h and labeled with FITC-BrdU; cell proliferation was examined by flow cytometry. The data represent three independent experiments. Error bars, mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.