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. 2018 Aug 15;10(8):2610–2618.

Figure 2.

Figure 2

LiCl induced cell cycle G2/M phase arrest. A, B. RPMI-8226 cells were treated with different concentrations (5 mM, 10 mM, 20 mM, and 40 mM) of LiCl for 48 h, with 40 mM NaCl used as a negative control. Then the cell cycle was detected by flow cytometry. C. Western blot analyses of cell cycle-associated proteins cyclin D1 and cyclin B1 in RPMI-8226 cells treated with 40 mM LiCl, β-actin was used as internal reference. D, E. Cell cycle analysis of U266 cells treated with different doses (5 mM, 10 mM, 20 mM, and 40 mM) of LiCl for 48 h, and the distribution of the subpopulation was analyzed based on three independent experiments. F. Cell cycle-associated proteins cyclin D1 and cyclin B1 were detected by Western blot in U266 cells treated with 40 mM LiCl for 48 h. All experiments were conducted at least three times. Error bars, mean ± SD. *, P < 0.05; **, P < 0.01.