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. 2018 Aug 15;10(8):2610–2618.

Figure 5.

Figure 5

The Wnt/β-catenin signaling pathway was activated in MM cells. A, B. Western blot analyses of the GSK-3β and p-GSK-3β protein levels in RPMI-8226 and U266 cells treated with 40 mM NaCl or 40 mM LiCl for 48 h, and using β-actin as an internal reference. C, D. RPMI-8226 and U266 cells were treated with 40 mM NaCl or 40 mM LiCl, the nuclear proteins were extracted, and then Western blot analyses of β-catenin and p-β-catenin protein levels were performed, using histone H3 as an internal reference. E, F. TOP/FOP Flash luciferase reporter assay indicating the activity of the downstream transcriptional factor of the Wnt pathway in HEK293T and RPMI-8226 cells following 40 mM LiCl treatment. Error bars, mean ± SD. *, P < 0.05.