Helix dynamics illustrated by separated local-field PISEMA spectra. (A) Data and PISA wheel for GW5,19ALP23 are shown. (B) The PISA wheel from (A) is repeated along with data showing extensive motional averaging for W2,22W5,19ALP23 (blue contours) and for GW4,20ALP23 (black contours). Each sample is oriented in DMPC/DHoPC bicelles with β = 90° and is 15N-labeled in residues 13–17 (assigned as indicated). The spectra for GW5,19ALP23 and W2,22W5,19ALP23 were recorded previously (17, 18). To see this figure in color, go online.