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. 2018 Jun 7;114(11):2717–2731. doi: 10.1016/j.bpj.2018.04.036

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Experimental AFM indentation responses on heterogeneous PDMS samples with SOFT (PDMS-B layered on PDMS-A) and STIFF (PDMS-A layered on PDMS-B) bilayer configurations. (A) Representative indentation force versus depth and (B) the resulting pointwise elastic modulus versus depth curves are shown. (C) Corresponding elastic modulus versus effective inclusion volume fraction is reconstructed using the FEM-generated master curve for each sample configuration. Solid lines represent the best-fit Eshelby curve for each data set, yielding E_sub at f_eff = 0 and E_inc at f_eff = 1. (D) Mean (± SD, n = 6–8) elastic properties of PDMS-A and PDMS-B obtained by HED analysis of AFM indentation of SOFT (squares) and STIFF (triangles) heterogeneous bilayer samples are shown as well as homogeneous samples of PDMS-A and PDMS-B (diamonds). Note: the data ranges of elastic moduli corresponding to the selected volume fraction were indicated by up arrows (SOFT) and down arrows (STIFF). To see this figure in color, go online.