Figure 9.

(A) Representative AFM indentation response of mouse aorta showed that indentation of the RING configuration yielded a stiffer measurement than that of the FLAT sample. Distributions show apparent elastic properties from arrays of individual AFM indentations of (B) RING and FLAT sections and (C) the deconstructed intima and media properties extracted from the composite FLAT sections using HED analysis. (D) Mouse aorta elastic properties obtained from AFM indention on RING and FLAT configurations are shown along with the layer-specific elastic moduli of the intima and media obtained by HED analysis of E_flat. Of note, the deconstructed medial layer, E_med, was not significantly different from the directly measured E_ring value.