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. 2018 Sep 3;12:276. doi: 10.3389/fncel.2018.00276

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Copyright © 2018 Laing, Li, Schmidt, Bunner, Yuan, Landry, Prete, McClung and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fasting induces cFOS and phosphorylation of ERK1/2 in AgRP/NPY neurons. (A) Representative images of cFOS (red) and NPY/AgRP neurons (green) under fed (n = 3) and fasted (n = 3) conditions. Count data for mean NPY/AgRP neurons per slice and mean number of AgRP/neurons co-localized with cFOS. (B) Representative images of pERK (red) and NPY/AgRP neurons (green) under fed and fasted conditions. Count data for mean NPY/AgRP neurons per slice and mean number of AgRP/NPY neurons co-localized with pERK. Bar graphs show Mean + SEM. *p < 0.05. Scale bars represent 50 μM.