Figure 3.
Fasting induces cFOS and phosphorylation of ERK1/2 in AgRP/NPY neurons. (A) Representative images of cFOS (red) and NPY/AgRP neurons (green) under fed (n = 3) and fasted (n = 3) conditions. Count data for mean NPY/AgRP neurons per slice and mean number of AgRP/neurons co-localized with cFOS. (B) Representative images of pERK (red) and NPY/AgRP neurons (green) under fed and fasted conditions. Count data for mean NPY/AgRP neurons per slice and mean number of AgRP/NPY neurons co-localized with pERK. Bar graphs show Mean + SEM. *p < 0.05. Scale bars represent 50 μM.