Figure 6.

Antiandrogen therapy inhibits IL10 production. Rats were treated with flutamide (7.5 mg/kg/day) for 5 days and thioglycollate-elicited peritoneal cells were pulsed ex vivo with LPS 1 μg/ml for 24 h. (A) Immunofluorescence for IL10 in absence of antiandrogens, analyzed by confocal microscopy. Most of Gr (+) peritoneal neutrophils express IL10. Bar = 50 μm. (B) Flow cytometry showing a decrease in the granulocytic population (R1) along with an increase in the frequency of mononuclear cells (R2) after flutamide treatment (representative dot plots; n = 3; top panels). The Gr (+) CD11b (+) R1 neutrophil population shows a decrease in IL10 expression after flutamide treatment (center panels, left) while the monocytic R2 population also displays low expression of IL10 in the same group (bottom panels). Data are representative of n = 3.