Table 4.
Hippocampal gene expression of Sham, OvxC, OvxL, and OvxF animals.
| Genes | Groups | |||
|---|---|---|---|---|
| Sham | OvxC | OvxL | OvxF | |
| 5-HT1A | 1.02 ± 0.12 | 0.96 ± 0.15 | 0.91 ± 0.09 | 0.80 ± 0.08 |
| 5-HT1B | 1.04 ± 0.17 | 1.05 ± 0.06 | 1.16 ± 0.06 | 0.96 ± 0.10 |
| 5-HT2C | 1.00 ± 0.04 | 0.98 ± 0.16 | 1.50 ± 0.27 | 1.35 ± 0.19 |
| 5-HTT | 1.20 ± 0.40 | 0.79 ± 0.13 | 0.78 ± 0.18 | 0.76 ± 0.09 |
| POMC | 1.01 ± 0.10 | 1.43 ± 0.21 | 0.90 ± 0.09 | 1.36 ± 0.12 |
| CART | 1.07 ± 0.23 | 1.17 ± 0.21 | 1.15 ± 0.25 | 1.14 ± 0.22 |
| NPY | 1.00 ± 0.01 | 0.95 ± 0.09 | 1.09 ± 0.13 | 0.99 ± 0.06 |
| AgRP | 1.01 ± 0.10 | 0.88 ± 0.08 | 0.89 ± 0.09 | 1.12 ± 0.10 |
| AdipoR1 | 1.00 ± 0.01 | 0.94 ± 0.02 | 1.09 ± 0.08 | 1.14 ± 0.11 |
| AdipoR2 | 1.00 ± 0.04 | 0.83 ± 0.04 | 0.94 ± 0.10 | 0.77 ± 0.05 |
| ObR | 1.02 ± 0.12 | 1.06 ± 0.17 | 1.21 ± 0.20 | 1.02 ± 0.02 |
The mRNA levels were measured by RT-PCR and expression of the target genes was normalized against that of the Sham animals.
Values are mean ± SEM n = 5–6. The housekeeping gene β-actin mRNA was used to normalize the relative amounts of mRNA.