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. 2018 Sep 3;9:1229. doi: 10.3389/fpls.2018.01229

FIGURE 3.

FIGURE 3

The alignment of a part of the HORVU7Hr1G030250 genomic and cDNA gene sequences representing exon 6th, intron 6th, and exon 7th of rhl1.b mutant and its parent variety ‘Karat’. The mutant allele harbors a single base-pair change A>T located in acceptor splice sites and is highlighted by a red color. Blue color represent the premature STOP codon in the gene sequence in the mutant. Green color indicates the correct STOP codon in the gene of cv. ‘Karat’. gDNA, genomic sequence; cDNA, transcript sequence. All nucleotides positions are related to the start codon in HORVU7Hr1G030250 sequence. The part of exon 7th (223 bp long and identical between both genotypes) is not shown.