Fig. 9.

Nitrite-dependent NO formation in liver, heart, aorta, and blood. (A) Curves (i), (ii), (iii) and (iv) show the nitrite-dependent NO formation by liver, heart, aorta and blood, respectively (1 g of tissue or 5 mL of blood with 100 μM nitrite). (B) The contribution of or complex I (ii), cytochrome P₄₅₀ (iii), XOR (iv), AO (v) and XOR plus AO (vi) to the measured NO, in liver and heart, was accessed using the inhibitors rotenone (ii), clotrimazole (iii), oxypurinol (iv), raloxifene (v) and oxypurinol plus raloxifene (vi), respectively (comparatively to an assay without inhibitors (i)). The general enzymatic contribution was accessed by the decreased NO formation caused by preheated the tissue (liver or heart) at 95 °C for 5 min (vii). NO measurements were performed using a chemiluminescence NO analyzer. *, significant inhibition, p < 0.05, compared with the respective control (bar i). Adapted from reference 163 with permission.