Fig. 4.

Inhibition of mPTP opening prevents oxidative stress and mitochondrial superoxide levels in fibroblasts of AD patients. A. Representative fluorescent images of DCF intensity before and after treatment with CsA. Bar= 10 µm B. Representative fluorescent images of MitoSOX intensity after treatment with CsA. Bar= 10 µm C. Graph showing quantitative data of ROS levels for all fibroblasts. Data are represented as mean ± SE; n = 3 technical replicates for each subject. *p < 0.05 indicates differences with control patients, #p < 0.05 indicates differences between control and CsA treatment calculated by Student's t-test. D. Graph showing quantitative data of superoxide levels in each patient's cell type. Data are represented as mean ± SE; n = 3 technical replicates for each subject. *p < 0.05 indicates differences between AD and control patients, #p < 0.05 indicates differences between control and CsA treatment calculated by Student's t-test.