Table 1.
Interday and intraday CV values for 11 LSD enzyme activities using LC-MS/MS.
| Sample | Assay procedure | GLA |
GAA |
IDUA |
ABG |
ASM |
GALC |
ID2S |
GALN |
ARSB |
NAGLU |
GUSB |
n | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CV (%) | ||||||||||||||
| Intraday | Low | 11-plex | 7 | 7 | 7 | 8 | 17 | 19 | 20 | 14 | 8 | 9 | 7 | 7 |
| Low | 6/3/2-plex | 12 | 7 | 4 | 7 | 16 | 6 | 17 | 14 | 9 | 9 | 6 | 7 | |
| High | 11-plex | 6 | 3 | 5 | 5 | 12 | 8 | 11 | 5 | 12 | 5 | 4 | 7 | |
| High | 6/3/2-plex | 5 | 5 | 9 | 7 | 13 | 10 | 16 | 15 | 6 | 9 | 5 | 7 | |
| Interday | Low | 11-plex | 10 | 13 | 15 | 21 | 22 | 4 | 10 | 10 | 14 | 12 | 9 | 3 |
| Low | 6/3/2-plex | 15 | 12 | 2 | 25 | 14 | 11 | 23 | 14 | 20 | 11 | 6 | 3 | |
| High | 11-plex | 2 | 8 | 8 | 2 | 4 | 7 | 6 | 2 | 5 | 10 | 11 | 3 | |
| High | 6/3/2-plex | 4 | 7 | 7 | 6 | 15 | 8 | 6 | 3 | 1 | 11 | 2 | 3 | |
Low, QC DBS with 5% enzyme activity; High, QC DBS with 100% enzyme activity.