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Hepatic protein expression of total IRS-1 (tIRS-1) (a), total IRS-2 (tIRS-2) (b), pSer307 in IRS-1 (c), and pSer731 in IRS-2 (d) in LETO-C, OLETF-C, and OLETF-RSG groups. Protein expression was determined by Western blot. The result in the LETO-C group was arbitrarily assigned a value of 1. Data are means ± SD (n = 8 each group) versus OLETF group (∗P < 0.05).
