Figure 4. Sequence analysis of the regulatory regions.

1. Distribution of observed phenotypes of mutant circuits with mutations in the regulatory region of the “green” gene. Phenotypes are colour‐coded (legend). Smaller graphs to the right indicate the subset of networks that have mutations only in the promoter or only in the operator. The data are based on 73 and 67 mutants of the opposing and concurring gradients networks, respectively.
2. Wild‐type sequences of regulatory regions (top of each panel, important elements labelled and coloured) together with the number of mutations at each site of a regulatory region that produce phenotypes of a given kind (bar‐charts below sequence, phenotypes labelled and colour‐coded). The height of each bar corresponds to the number of mutant networks with a mutation at a given position, where these mutations produced the indicated phenotype. Only phenotypes produced by at least three mutant circuits are shown. Red arrows indicate genotypes that can produce a novel phenotype with a single mutation at the indicated position.