Extended Data Table 1.
Cryo-EM data collection, refinement and model validation statistics.
| Orco-Fab | |
|---|---|
| Data deposition | |
| PDB | 6C70 |
| EMDB | 7352 |
|
| |
| Cryo-EM Data Collection | |
| Voltage (kV) | 300 |
| Magnification (×) | 29,000 |
| Pixel size (Å) | 1.0 |
| Electron exposure (e−/Å2/frame) | 1.6 |
| Defocus range (μm) | −0.8 to −2.0 |
| Number of image stacks | 1,221 |
| Number of frames per stack | 50 |
|
| |
| Cryo-EM Data Processing | |
| Initial number of particles | 82,614 |
| Final number of particles | 53,141 |
| Symmetry imposed | C4 |
| Map sharpening B factor (Å2) | −160 |
| Map resolution (Å) | 3.5 |
| Map resolution range (Å) | 3–8 |
| FSC threshold | 0.143 |
|
| |
| Model Refinement | |
| Number of Orco amino acids | 388 |
| Number of Fab amino acids | 0 |
| Total non-hydrogen atoms | 3092 |
| Average B factor (Å2) | 104 |
| Bond length r.m.s.d. (Å) | 0.01 |
| Bond angle r.m.s.d. (°) | 1.07 |
| Ramachandran Plot: | |
| Favoured (%) | 97.4 |
| Allowed (%) | 2.6 |
| Outliers (%) | 0 |
| Rotamer outliers (%) | 0 |
| MolProbity score | 1.7 |
| MolProbity clash score | 7.6 |