Figure 7.

GNC enhances chloroplast biogenesis by inhibiting expression of target genes involved in brassinosteroid biosynthesis and signaling. BR-activated transcription factors encoded by BES1, BEH2, BEH3, BEH4, BIM1, and enzymes involved in BR biosynthesis encoded by STE1/DWF7, DWF3, and DWF4 were characterized. A, GNC ChiP-seq binding profiles based on IGB viewer. Fold enrichment for each sample (IP/input) was computed by MACS using bdgcmp. The range starts at 1 (IP = input), and the maximum varies per panel (5 for BES1, BEH3, and DWF4; 4 for DWF3; 3.5 for BEH2 and BIM1; 3 for BEH4 and STE1). B, Gene expression determined by RT-qPCR on 6-d-old green seedlings for two independent DEX-inducible GNC lines, as well as wild-type, gnc cga1, and two independent 35S:GNC lines. Statistical analysis was performed as in Figure 6.