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. 2018 Jul 12;178(1):130–147. doi: 10.1104/pp.18.00414

Figure 8.

Figure 8.

The GNC family regulates stomatal development in cotyledons. A, Effect on stomata index (SI), meristemoid index (MI), and stomata lineage index (SLI) in cotyledons of Col-0, gnc cga1, and two independent 35S:GNC lines. Parameters were determined for 3-d-old (top), 6-d-old (middle), and 10-d-old seedlings (bottom). For statistical analysis, one-way ANOVA with post-hoc Holm multiple-comparison calculation was used to compare the mutant lines to the wild type (*P < 0.05, **P < 0.01, n = 10). Error bars indicate ± sd. B, GNC ChiP-seq binding profiles based on IGB viewer. Fold enrichment for each sample (IP/input) was computed by MACS using bdgcmp. The range starts at 1 (IP = input), and the maximum varies per panel (4 for ERL1 and ERL2; 3 for EPF2 and EPFL6; 2.5 for SPCH). C, GNC regulates expression of genes involved in stomatal development and patterning. Gene expression determined by RT-qPCR on two independent DEX-inducible GNC lines, as well as wild-type, gnc cga1, and two independent 35S:GNC lines. Statistical analysis was performed as in Figure 6.