Figure 3.

Mutation of RAPTOR1B leads to decreased accumulation of ROS after exposure to light. A, Fluorescence images of ROS (as indicated by H₂DCFDA) and chlorophyll in the cotyledons of 5-d-old etiolated seedlings followed by 2 d of white light. Bars = 200 µm. B, Analysis of ROS-responsive gene expression by RT-qPCR of 5-d-old etiolated seedlings after exposed to white light for 1 h. The expression level of UBQ10 was used as an internal standard. Error bars represent sd (n = 3). C, Singlet oxygen (as indicated by SOSG) production in the cotyledons of 5-d-old etiolated seedlings. Bars = 200 µm.