Figure 6.

rb seedlings show considerably altered primary metabolism. Histograms show the relative levels of metabolites extracted from Col-0, rb10, and rb22 seedlings. A, Glu is an essential precursor for tetrapyrrole biosynthesis. B and C, Tricarboxylic acid cycle intermediates (B) and carbohydrates from primary metabolism (C). All seedlings were grown in the dark for 5 d and then transferred to the light. Samples were collected immediately prior to light exposure (5 d of dark) or after defined periods of light exposure (6, 12, or 24 h of light). For each genotype and time point, three independent biological samples were harvested. The histograms show means of three replicates ± sd (n = 3). Asterisks represent values that are statistically different from that of the wild-type sample at the same condition (*, P < 0.05 and **, P < 0.01 by Student’s t test). The normalized data of all measured primary metabolites can be obtained from Supplemental Table S1. AU, Arbitrary units; WT, wild type.