Figure 4.

MS188 but not AMS activates the expression of ACOS5, CYP704B1, and TKPR1. A to C, The designed probes in the promoter and genomic regions of TKPR1, ACOS5, and CYP704B1, respectively. D to F, The fragments of the above probes in TKPR1, ACOS5, and CYP704B1 promoters were specifically amplified by ChIP-qPCR using 4xMYC-MS188 transgenic plant samples. The fold enrichment is the average of three replicates (error bar is shown by sd). −AB, absence of antibody; +AB, presence of antibody. G to I, Transient dual-luciferase assays were performed in Arabidopsis leaf protoplasts. The p35S::NOS vector was used as negative control. The p35S::MS188 and p35S::AMS were transformed into the protoplasts with reporter plasmids separately. The last column of three panels was cotransformation of p35S::MS188, p35S::AMS, and each reporter plasmid. sd is indicated as error bar.