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. 2018 Jul 20;178(1):428–440. doi: 10.1104/pp.18.00683

Figure 5.

Figure 5.

The K283 and K165 residues in MYB30 are important for its degradation by the 26S proteasome. A, Degradation of MYB30, MYB30K165R, MYB30K283R, and MYB30K165/283R protein in Arabidopsis protoplasts. FLAG-MYB30, FLAG-MYB30K165R, FLAG-MYB30K283R, and FLAG-MYB30K165/283R plasmids were transiently expressed overnight in Arabidopsis protoplasts, which were then treated with 100 μm CHX and 75 μm MG132 for the indicated periods of time. ACTIN was used as a loading control. B, The FLAG-MYB30 protein levels in A were quantified. All protein levels were normalized to ACTIN and their initial value. Three independent biological replicates were analyzed. Data presented are means ± sd. C, ABA-induced MYB30, MYB30K165R, and MYB30K283R degradation in the siz1-2 mutant. Seedlings were treated with 100 μm ABA for 12 or 24 h. The MYB30 levels were detected by immunoblotting with an anti-FLAG antibody. ACTIN was used as a loading control. D, The protein levels in C were normalized to that of FLAG-MYB30 normalized to ACTIN at time 0. Three independent biological replicates were analyzed. Data presented are means ± sd.