Table 2. Leaf anatomical measurements in the wild type and αPEPC mutants of S. viridis.
Statistically significant differences according to posthoc Tukey’s test at P < 0.05 are indicated by different lowercase letters. Values followed by the same letter within a row are not significantly different. BS, Bundle sheath; M, mesophyll; M-M, mesophyll-mesophyll cell interface.
| Parameter | Wild Type | αPEPC 12 | αPEPC 13 | αPEPC 4 |
|---|---|---|---|---|
| BS cell wall thickness (µm) | 0.68 ± 0.029 a | 0.29 ± 0.019 b | 0.52 ± 0.026 c | 0.23 ± 0.009 b |
| M cell wall thickness (µm) | 0.27 ± 0.009 a | 0.11 ± 0.004 b | 0.22 ± 0.011 c | 0.12 ± 0.005 b |
| Sb (m2 m−2) | 2.38 ± 0.035 a | 2.33 ± 0.042 a | 2.32 ± 0.046 a | 2.06 ± 0.037 b |
| M-BS PD per pit field area (PD µm−2) | 50.8 ± 0.49 a | 61.6 ± 1.19 b | 55.7 ± 0.55 c | 51.0 ± 0.57 a |
| M-BS pit field area per interface area (%) | 12.9 ± 0.27 a | 16.4 ± 0.27 b | 18.9 ± 0.54 c | 22.9 ± 0.42 d |
| M-BS PD per interface area (PD µm−2) | 6.6 ± 0.12 a | 10.1 ± 0.13 b | 10.5 ± 0.10 b | 11.7 ± 0.17 c |
| M-BS average pit field area (µm2) | 0.6 ± 0.05 a | 0.5 ± 0.04 a | 0.7 ± 0.06 a | 0.6 ± 0.07 a |
| M-BS PD area (µm2) | 0.008 ± 0.0002 a | 0.008 ± 0.0002 a,b | 0.007 ± 0.0002 b | 0.005 ± 0.0002 c |
| M-BS PD area per interface area (µm2 µm−2) | 0.054 ± 0.0005 a | 0.079 ± 0.0007 b | 0.077 ± 0.0006 c | 0.061 ± 0.0007 d |
| M-BS PD area per unit of leaf area (m2 m−2) | 0.128 ± 0.0007 a | 0.184 ± 0.0010 b | 0.178 ± 0.0010 c | 0.125 ± 0.0009 a |
| M-M PD per pit field area (PD µm−1) | 55.0 ± 1.26 a | 62.9 ± 1.14 b | 55.0 ± 0.74 a | 71.1 ± 0.76 c |
| M-M pit field area per interface area (%) | 6.3 ± 0.15 a | 3.4 ± 0.10 b | 4.8 ± 0.12 c | 6.8 ± 0.16 a |
| M-M PD per interface area (PD µm−2) | 3.5 ± 0.04 a | 2.1 ± 0.02 b | 2.6 ± 0.04 c | 4.9 ± 0.08 d |
| M-M average pit field area (µm2) | 0.6 ± 0.10 a | 0.3 ± 0.03 b | 0.4 ± 0.04 a,b | 0.3 ± 0.04 b |
| M-M PD area (µm2) | 0.010 ± 0.0004 a | 0.006 ± 0.0002 b | 0.006 ± 0.0001 b | 0.004 ± 0.0001 c |