Analysis of plant NUE and seed N and C content of Arabidopsis aap2 and wild-type (WT) plants at harvest. Plants were grown in 16-h days for 8 weeks under low-, moderate-, or high-N conditions. A, Seed yield per plant (n ≥ 12). B, Plant NUE, calculated based on seed yield relative to N supply (n ≥ 12). C, N content of seeds in percentage (n = 6). D, C content of seeds in percentage (n = 6). E, Total seed N content per plant (n = 6). F, Total seed C content per plant (n = 6). Data are means ± sd. Significant differences are indicated by letters (ANOVA; P ≤ 0.05).