Figure 6.

The TGMV Rep phosphomimic S97D mutant does not infect tomato. Tomato Florida Lanai plants were inoculated by cobombardment of a wild-type TGMV-B replicon with a wild-type (wt) TGMV-A or an AL4-knockout (KO) mutant with wild-type Rep or the S97D or S97N Rep mutant. The mock controls were inoculated with the TGMV-B alone. A, Leaflets from plants at 24 dpi. B, Leaf chlorosis symptoms were scored on a scale of 1 to 5 at 10, 17, 24, 31, 38, and 45 dpi. The average symptom scores from 10 plants for each treatment are shown. C, The presence of TGMV DNA-A was assessed by PCR at 28 dpi using specific primers that yielded a 403-bp product. Results from two independent plants are shown for each treatment, except for mock.