Figure 8.

Relative transcript and metabolite abundance in stress-induced switchgrass leaf blade and root tissues. A and B, Analysis of relative transcript (A) and metabolite (B) abundance in stress-treated (blue) and control (gray) tissues. In the left column, detached leaf blades were treated with 20 min of UV irradiation (254 nm). In the right column, roots were treated by adding 10 mm CuSO₄ solution daily to the soil of switchgrass plants. RT-qPCR was used to measure relative transcript abundance and normalized to the internal reference gene elongation factor 1α (EF-1α). Relative metabolite abundance was determined by GC-MS analysis. Error bars represent propagated se of the mean (n = 3). Asterisks depict statistically significant differences between stress-treated and control samples based on Student’s t test (P < 0.05). DW, Dry weight.