Figure 1.

Extraction of the ER enrichment fraction from rice seeds. A, Coomassie Blue-stained gel showing the increase in glutelin precursors in ER-stressed rice seeds. M, Marker; WT, wild-type rice seeds; Ri, OsSAR1 RNAi rice seeds (the numbers represent different transgenic rice lines). B, A brief workflow of the extraction of the ER enrichment fraction from rice seeds. C, CNX, BIP1, PDI2-3, and glutelin subunits were detected by Coomassie Blue staining and immunoblotting. OE23 (the 23-kD subunits of the oxygen-evolving complex) serves as a marker protein of chloroplasts. Seeds indicates total proteins from rice seeds, and ER indicates proteins of the ER enrichment fraction.