Figure 2.

Importin Binding Is Not Required for MeCP2 Retention in the Nucleus

(A) NIH 3T3 cells expressing mutated forms of MeCP2 as EGFP fusion proteins and counterstained with DAPI. The NLS of MeCP2 is intact (G273X) or truncated (R255X), and the DNA binding domains are WT, impaired (ΔMBD), or severely impaired (ΔMBDΔhook 1). The scale bars represent 5 μm.

(B) Quantification of ratio of cytoplasmic to nuclear signal intensity of EGFP-MeCP2 in above panels. Data points represent individual cells. Horizontal bars represent means. Statistical significance was calculated using the Wilcoxon test.

(C) IP using an antibody against EGFP of EGFP-MeCP2, KPNA3, and KPNA4 from extracts of HeLa cells expressing full-length (FL), G273X, R270X, and R255X forms of EGFP-MeCP2.

See also Figure S1.