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. 2018 Sep 10;38:25. doi: 10.1186/s41232-018-0083-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Elongation of endothelial cells derived from ES cells. a In the co-culture system with OP9 stromal feeder cells, ES-derived endothelial cells form a round colony in the presence of a low level of VEGF, which produced by OP9 cells (left panel). These cells form long elongated structures, when stimulated by a high level of VEGF (right panel). Scale bar 200 μm. b ES-derived endothelial cells form vessel-like structures in the 3D collagen gel culture. Scale bar 100 μm