Skip to main content
. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: ACS Chem Biol. 2018 May 30;13(6):1551–1559. doi: 10.1021/acschembio.8b00154

Figure 1.

Figure 1.

Identification of TL02–59 as a potent inhibitor of AML cell growth. A) TL02–59 and nine N-phenylbenzamide analogs were evaluated for their growth inhibitory activity in the FLT3-ITD+ AML cell line, MV4–11, using the CellTiter Blue assay (Promega). IC50 values for each compound are shown, with the R-group unique to each analog plotted next to the corresponding data point. The structures of TL02–59 and the related TL8 scaffold are shown on the right. B) Proliferation of MV4–11, MOLM-14 and THP-1 AML cells in the presence of TL02–59 was measured using the CellTiter-Blue assay over a range of concentrations for 72 hours. For THP-1 cells, additional concentrations up to 3.0 μM did not significantly inhibit growth (not shown). C) MV4–11, MOLM-14 and THP-1 AML cells were incubated with tandutinib (100 nM), TL02–59 (100 nM), staurosporine (1 μM) or DMSO for 72 hours. Apoptosis and cell viability were independently measured and results are presented as mean ratios of caspase activity to cell viability from three independent experiments ± SD.