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. Author manuscript; available in PMC: 2019 Aug 20.
Published in final edited form as: Chem Res Toxicol. 2018 Jul 30;31(8):697–711. doi: 10.1021/acs.chemrestox.8b00055

Figure 6.

Figure 6.

Pol κ SNPs have similar nucleotide incorporation patterns extending from the correct base pair as WT pol κ (the templating base is dT). From both the (A) dG:dC and (B) N2ffdG:dC base pairs, there is only correct dA incorporation. (C) For the undamaged dA:dT control, dA incorporation is observed for all the variants, and the S423R variant incorporates multiple dAs and misincorporates every nucleotide, including multiple dGs. (D) In assays of incorporation after the N6ffdA:dT base pair, in which the template base is dG, no misincorporation by WT or any variant is observed; only correct dC incorporation is observed. With ethenodA:dT base pair, very little activity is observed. P = primer, 30 min time points; quantitation is reported in Table S6.