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. 2017 Apr 21;49(4):e319. doi: 10.1038/emm.2017.31

Figure 5.

Figure 5

Effects of FSK and CCH on intracellular calcium ion concentrations ([Ca2+]i) in bladder ICC-LCs. (a, b) Six pictures from each group of naive or CYP-treated WT and HCN1−/− mice were chosen to represent the continuous changes in real-time [Ca2+]i before and after drug (CCH, FSK, ZD7288) administration. CCH or FSK was applied after recording for 1 min, and ZD7288 was applied after recording for 2 min. (c, d) The effects of CCH and FSK on [Ca2+]i in each group were expressed as relative fluorescence intensities (RFI, F1/F0), which were illustrated as continuous curves. Application of CCH (1 μM) and FSK (50 μM) significantly enhanced [Ca2+]i in all four groups, while application of ZD7288 (50 μM) decreased [Ca2+]i in all four groups. HCN1 channel deletion significantly weakened the effects of CCH (e) or FSK (f) on [Ca2+]i in the bladder ICC-LCs of both naive and CYP-treated mice (data represent the mean±s.d., N=10, ***P<0.001, versus WT mice).